A Model of Peak Amplitude Enhancement in Orthogonal Two-Dimensional Gas Chromatography

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• 作者：Andrew L. Lee ; Keith D. Bartle ; and Alastair C. Lewis
• 刊名：Analytical Chemistry
• 出版年：2001
• 出版时间：March 15, 2001
• 年：2001
• 卷：73
• 期：6
• 页码：1330 - 1335
• 全文大小：73K
• 年卷期：v.73,no.6(March 15, 2001)
• ISSN：1520-6882

文摘

Increases in peak capacity that are achieved using comprehensive rather than single-column gas chromatographyare also often accompanied by significant improvementsin minimum detectable amounts. Comprehensive gaschromatography utilizes a zone compression of eluentbetween primary and secondary chromatography columns, with complete conservation of mass. In this paper,we present a theoretical model of peak amplitude enhancement based on translation of the fractional areaunder the primary column normal curve to a secondaryGaussian peak. Amplitude enhancement made by peakmodulation scales with increasing modulation period andprimary column peak width. An exponential relationshipbetween the modulated secondary column peak width andthe amplitude enhancement is also observed. The calculated amplitude gains are further corrected for detectorcurrent noise generated via flame ionization detection ata range of operating frequencies. An example of trace levelatmospheric analysis is used to demonstrate both peakcapacity and sensitivity enhancements using the multidimensional technique.