Studies on Antioxidant Activity of Pomegranate (Punica granatum) Peel Extract Using in Vivo Models

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• 作者：Kotamballi N. Chidambara Murthy ; Guddadarangavvahally K. Jayaprakasha ; and Ravendra P. Singh
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2002
• 出版时间：August 14, 2002
• 年：2002
• 卷：50
• 期：17
• 页码：4791 - 4795
• 全文大小：143K
• 年卷期：v.50,no.17(August 14, 2002)
• ISSN：1520-5118

文摘

Pomegranate (Punica granatum) peel extracts have been shown to possess significant antioxidantactivity in various in vitro models. Dried pomegranate peels were powdered and extracted withmethanol for 4 h. The dried methanolic extract was fed to albino rats of the Wistar strain, followed bycarbon tetrachloride (CCl₄), and the levels of various enzymes, such as catalase, peroxidase, andsuperoxide dismutase (SOD), and lipid peroxidation were studied. Treatment of rats with a singledose of CCl₄ at 2.0 g/kg of body weight decreases the levels of catalase, SOD, and peroxidase by81, 49, and 89% respectively, whereas the lipid peroxidation value increased nearly 3-fold.Pretreatment of the rats with a methanolic extract of pomegranate peel at 50 mg/kg (in terms ofcatechin equivalents) followed by CCl₄ treatment causes preservation of catalase, peroxidase, andSOD to values comparable with control values, wheres lipid peroxidation was brought back by 54%as compared to control. Histopathological studies of the liver were also carried out to determine thehepatoprotection effect exhibited by the pomegranate peel extract against the toxic effects of CCl₄.Histopathological studies of the liver of different groups also support the protective effects exhibitedby the MeOH extract of pomegranate peel by restoring the normal hepatic architecture.Keywords: Punica granatum; reactive oxygen species; antioxidant activity; lipid peroxidation; SOD;peroxidase; catalase