Dual Labeled Peptides as Tools to Study Receptors: Nanomolar Affinity Derivatives of TIPP (Tyr-Tic-Phe-Phe) Containing an Affinity Label and Biotin as Probes of δ Opioid Receptors

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• 作者：Jane V. Aldrich* ; Vivek Kumar^† ; ^§ ; ; Thomas F. Murray^‡ ; ; Wei Guang^† ; ; Jia Bei Wang^† ;
• 刊名：Bioconjugate Chemistry
• 出版年：2009
• 出版时间：February 18, 2009
• 年：2009
• 卷：20
• 期：2
• 页码：201-204
• 全文大小：165K
• 年卷期：v.20,no.2(February 18, 2009)
• ISSN：1520-4812

文摘

A general strategy for the design of dual labeled peptides was developed, and derivatives of the δ opioid receptor (DOR) selective antagonist TIPP (Tyr-Tic-Phe-PheOH) containing both an affinity label and biotin were prepared by solid-phase synthesis. Tyr-Tic-Phe-Phe(p-X)-Asp-NH(CH₂CH₂O)₂-CH₂CH₂NH-biotin (where X = NCS or NHCOCH₂Br) exhibit nanomolar DOR affinity. The ability to detect receptors labeled with these peptides following solubilization and SDS-PAGE demonstrate the applicability of this design approach for dual labeled peptide derivatives.