PLGA:Poloxamer and PLGA:Poloxamine Blend Nanoparticles: New Carriers for Gene Delivery
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- 作者：Noé ; mi Csaba ; Pilar Caamañ ; o ; Alejandro Sá ; nchez ; Fernando Domí ; nguez ; and Marí ; a José ; Alonso
- 刊名：Biomacromolecules
- 出版年：2005
- 出版时间：January 2005
- 年：2005
- 卷：6
- 期：1
- 页码：271 - 278
- 全文大小：339K
- 年卷期：v.6,no.1(January 2005)
- ISSN：1526-4602
文摘
The main objective of the present work was the development of new nanoparticulate carrier systems for thedelivery of plasmid DNA. These new carriers consist of a blend matrix formed by a poly(lactic-co-glycolicacid) (PLGA) copolymer and polyoxyethylene derivatives. More specifically, we have prepared nanostructureswith different PLGA:poloxamer and PLGA:poloxamine compositions by an optimized emulsification-solvent diffusion technique and studied the potential of these carriers for the encapsulation and controlledrelease of plasmid DNA. Depending on the particle composition, the encapsulation efficiency of the modelplasmid pEGFP-C1 varied between 30% and 45%. All formulations provided continuous and controlledrelease of the plasmid with minimal burst effect. In addition, the release rate and duration was dependenton the composition of the particle matrix. Moreover, gel electrophoresis and cell culture (MCF-7 cell line)assays allowed us to confirm that the biologically active form of the plasmid was preserved during theparticle preparation process and also during its release. Cell culture experiments also indicated that the newnanoparticles do not exhibit toxic effects on these cells at concentrations up to 5 mg/mL. Altogether, theseresults indicate that these composite nanostructures present a promising approach for the delivery of plasmidDNA.