RNA Aptamer against Autoantibodies Associated with Multiple Sclerosis and Bioluminescent Detection Probe on Its Basis

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• 作者：Maria A. Vorobjeva ; Vasilisa V. Krasitskaya ; Alesya A. Fokina ; Valentina V. Timoshenko ; Georgy A. Nevinsky ; Alya G. Venyaminova ; Ludmila A. Frank
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：March 4, 2014
• 年：2014
• 卷：86
• 期：5
• 页码：2590-2594
• 全文大小：257K
• 年卷期：v.86,no.5(March 4, 2014)
• ISSN：1520-6882

文摘

Nowadays, there are no specific laboratory tests for establishing the diagnosis of multiple sclerosis (MS). The presence of proteolytic autoantibodies against myelin basic protein is now considered as a characteristic feature of MS. New 2鈥?F-containing RNA aptamer of high affinity and specificity to these antibodies was selected. Covalent conjugate of this aptamer and Ca²⁺-regulated photoprotein obelin was obtained for the first time and applied as a label in bioluminescent microplate assay to detect target antibodies. The developed model solid-phase microassay is simple, fast, and highly sensitive.