Evaluating Cytotoxicity and Cellular Uptake from the Presence of Variously Processed TiO2 Nanostructured Morphologies

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• 作者：Jingyi Chen ; Hongjun Zhou ; Alexander C. Santulli ; Stanislaus S. Wong
• 刊名：Chemical Research in Toxicology
• 出版年：2010
• 出版时间：May 17, 2010
• 年：2010
• 卷：23
• 期：5
• 页码：871-879
• 全文大小：474K
• 年卷期：v.23,no.5(May 17, 2010)
• ISSN：1520-5010

文摘

We evaluated the cytotoxicity of various morphological classes of TiO₂ nanostructures (including 0-D nanoparticles, 1-D nanorods, and 3-D assemblies) toward living cells. These TiO₂ nanostructures were modified with fluorescent dye molecules, mediated via a dopamine linkage, in order to facilitate a confocal study of their internalization. Specifically, we noted that both TiO₂ 1-D nanorods and 0-D nanoparticles could internalize into cells after 24 h of incubation time. However, only incubation with TiO₂ 1-D nanorods and 3-D micrometer-scale sea urchin-like assemblies at concentrations of up to 125 μg/mL yielded data suggestive of cell viabilities of close to 100%. Moreover, upon irradiation with UV light for periods of a few minutes at energy densities of up to 1 J/cm², we observed up to 60% mortality rates, indicative of the cytotoxic potential of photoirradiated TiO₂ nanostructures due to the generation of reactive oxygen species.