Absorption, Uptake and Tissue Affinity of High-Molecular-Weight Hyaluronan after Oral Administration in Rats and Dogs

详细信息    查看全文

• 作者：Lajos Balogh ; Andras Polyak ; Domokos Mathe ; Reka Kiraly ; Juliana Thuroczy ; Marian Terez ; Gyozo Janoki ; Yaoting Ting ; Luke R. Bucci ; Alexander G. Schauss
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：November 26, 2008
• 年：2008
• 卷：56
• 期：22
• 页码：10582-10593
• 全文大小：357K
• 年卷期：v.56,no.22(November 26, 2008)
• ISSN：1520-5118

文摘

The purpose of this study was to determine the absorption, distribution and excretion of ^99mtechnetium-labeled, high-molecular-weight hyaluronan ((^99mTc-HA) and ^99mtechnetium pertechnetate (^99mTc-P) after single dose, oral administration to Wistar rats and Beagle dogs. A pilot study utilized ^99mTc-HA alone, and a second confirmatory study compared uptake of labeled ^99mTc-HA with ^99mTc-P. Urinary and fecal excretion after ^99mTc-HA ingestion by rats showed 86.7−95.6% of radioactivity was recovered, almost all in feces. All tissues examined showed incorporation of radioactivity from ^99mTc-HA starting at 15 min and persisting for 48 h, in a pattern significantly different from ^99mTc-P. Whole-body scintigraphs and close-ups of the ventral chest region showed nonalimentary radioactivity from ^99mTc-HA concentrated in joints, vertebrae and salivary glands four hours after administration. Autoradiography of skin, bone and joint tissue pieces after 24 h showed incorporation of radioactivity from ^99mTc-HA, but not from ^99mTc-P. Conversely, absorption, distribution and excretion of ^99mTc was completely different from ^99mTc-HA, showing an expected pattern of rapid absorption and excretion in urine, with accumulation in thyroid glands, stomach, kidney and bladder. This report presents the first evidence for uptake and distribution to connective tissues of orally administered, high-molecular-weight HA.