Thi
s paper
summarize
s the recent activitie
s of the Chromo
some-Centric Human Proteome Project (C-HPP) con
sortium, which develop
s new technologie
s to identify yet-to-be annotated protein
s (termed 鈥渕i
ssing protein
s鈥? in biological
sample
s that lack
sufficient experimental evidence at the protein level for confident protein identification. The C-HPP al
so aim
s to identify new protein form
s that may be cau
sed by genetic variability, po
st-tran
slational modification
s, and alternative
splicing. Proteogenomic data integration form
s the ba
si
s of the C-HPP鈥檚 activitie
s; therefore, we have
summarized
some of the key approache
s and their role
s in the project. We pre
sent new analytical technologie
s that improve the chemical
space and lower detection limit
s coupled to bioinformatic
s tool
s and
some publicly available re
source
s that can be u
sed to improve data analy
si
s or
support the development of analytical a
ssay
s. Mo
st of thi
s paper鈥檚 content ha
s been compiled from po
ster
s,
slide
s, and di
scu
ssion
s pre
sented in the
serie
s of C-HPP work
shop
s held during 2014. All data (po
ster
s, pre
sentation
s) u
sed are available at the C-HPP Wiki (
sting.rug.nl/" class="extLink">http://c-hpp.webhosting.rug.nl/) and in the Supporting Information.