Quantification of Polymer Interactions in Bacterial Adhesion

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• 作者：Barbara A. Jucker ; Alexander J. B. Zehnder ; and Hauke Harms
• 刊名：Environmental Science & Technology
• 出版年：1998
• 出版时间：October 1, 1998
• 年：1998
• 卷：32
• 期：19
• 页码：2909 - 2915
• 全文大小：206K
• 年卷期：v.32,no.19(October 1, 1998)
• ISSN：1520-5851

文摘

Adhesion of bacteria to solids is governed by van derWaals, electrostatic, and acid-base (hydrophobic)interactions, which are combined in an extended DLVOmodel (DLVO-AB) and by interactions of bacterial surfacepolymers with the solid surfaces. A method to calculatepolymer interactions was not available yet, and their existencehad been inferred only qualitatively from the deviation ofthe actual adhesion from DLVO-AB-based expectations. Here,we present attempts (i) to quantify polymer interactionsfrom this deviation and (ii) to calculate them independentlyas the sum of repulsive and attractive contributions.Repulsion was assumed to result from the resistance ofthe polymer layer against compression. Its calculation wasmost sensitive to the packing density of the polymers inthe cell envelope. Attraction was assumed to origin frompolymer adsorption to the surface and was calculatedon the basis of adsorption data of isolated surface polymers.Comparison of total interaction energy curves withadhesion of six Gram-negative bacteria to glass showedthat the low adherence of five strains may have resulted fromdominant polymer repulsion, i.e., hardly compressiblecell envelopes hindered the bacteria to approach the energyminima resulting from DLVO-AB interactions. Onestrain adhered readily, possibly because polymer repulsionwas low and polymer attraction and DLVO-AB forcesdominated the overall interaction.