Bacterial Deposition in Porous Media Related to the Clean Bed Collision Efficiency and to Substratum Blocking by Attached Cells
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- 作者：Huub H. M. Rijnaarts ; Willem Norde ; Edward J. Bouwer ; Johannes Lyklema ; and Alexander J. B. Zehnder
- 刊名：Environmental Science & Technology
- 出版年：1996
- 出版时间：October 1996
- 年：1996
- 卷：30
- 期：10
- 页码：2869 - 2876
- 全文大小：662K
- 年卷期：v.30,no.10(October 1996)
- ISSN：1520-5851
文摘
Deposition of Pseudomonas putida mt2 andRhodococcusstrain C125 during transport through columns packedwith Teflon grains was investigated. Deposition wasanalyzed in terms of the clean bed collision efficiency
0 (the probability of a cell to attach uponreachinga cell-free substratum) and the surface area blockedby attached cells. Blocking was quantified by ablockingfactor B, the ratio of the blocked area per celltothe geometric area of a cell. At an averageinterstitialfluid velocity of 200 µm s-1,0 is close to unity (0.83± 0.01) for both strains, indicating that cell-solidinteractions are almost completely favorable for deposition. Values for B of 1.6 ± 0.1 and 12.0 ± 0.8wereobtained for Ps. putida and RhodococcusstrainC125, respectively. This difference is consistentwithdifferences in cell size and cell-cell repulsion, whichwere both smaller for Ps. putida than forRhodococcusstrain C125. At coverages close to saturation, multilayer adhesion and/or pore clogging occurs for theweakly blocking Pseudomonas cells but not forthestrongly blocking Rhodococcus cells. Thecollision-blocking concept succesfully explains the breakthrough of Bacillus cells in coarse sand columns,asreported by Lindqvist and Enfield, for which adhesionis less favorable (0 = 0.10 ± 0.01) and blockingisrelatively strong (B = 5.8 ± 0.8). Thegeneralconclusion is that deposition of microbes during theirtransport through coarse grain media is adequatelydescribed by the collision-blocking model in cases ofstrongly blocking cells or weakly blocking cells atlow coverage conditions.
0 (the probability of a cell to attach uponreachinga cell-free substratum) and the surface area blockedby attached cells. Blocking was quantified by ablockingfactor B, the ratio of the blocked area per celltothe geometric area of a cell. At an averageinterstitialfluid velocity of 200 µm s-1,0 is close to unity (0.83± 0.01) for both strains, indicating that cell-solidinteractions are almost completely favorable for deposition. Values for B of 1.6 ± 0.1 and 12.0 ± 0.8wereobtained for Ps. putida and RhodococcusstrainC125, respectively. This difference is consistentwithdifferences in cell size and cell-cell repulsion, whichwere both smaller for Ps. putida than forRhodococcusstrain C125. At coverages close to saturation, multilayer adhesion and/or pore clogging occurs for theweakly blocking Pseudomonas cells but not forthestrongly blocking Rhodococcus cells. Thecollision-blocking concept succesfully explains the breakthrough of Bacillus cells in coarse sand columns,asreported by Lindqvist and Enfield, for which adhesionis less favorable (0 = 0.10 ± 0.01) and blockingisrelatively strong (B = 5.8 ± 0.8). Thegeneralconclusion is that deposition of microbes during theirtransport through coarse grain media is adequatelydescribed by the collision-blocking model in cases ofstrongly blocking cells or weakly blocking cells atlow coverage conditions.