Live-Cell One- and Two-Photon Uncaging of a Far-Red Emitting Acridinone Fluorophore
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- 作者：David Warther ; Frdric Bolze ; Jrmie Lonard ; Sylvestre Gug ; Alexandre Specht ; David Puliti ; Xiao-Hua Sun ; Pascal Kessler ; Yves Lutz ; Jean-Luc Vonesch ; Barbara Winsor ; Jean-Franois Nicoud ; Maurice Goeldne
- 刊名：Journal of the American Chemical Society
- 出版年：2010
- 出版时间：March 3, 2010
- 年：2010
- 卷：132
- 期：8
- 页码：2585-2590
- 全文大小：205K
- 年卷期：v.132,no.8(March 3, 2010)
- ISSN：1520-5126
文摘
Total synthesis and photophysical properties of PENB-DDAO, a photoactivatable 1,3-dichloro-9,9-dimethyl-9H-acridin-2(7)-one (DDAO) derivative of a far-red emitting fluorophore, are described. The photoremovable group of the DDAO phenolic function comprises a donor/acceptor biphenyl platform which allows an efficient (≥95%) and rapid (<15 μs time-range) release of the fluorescent signal and displays remarkable two-photon uncaging cross sections (δa·Φu = 3.7 GM at 740 nm). PENB-DDAO is cell permeable as demonstrated by the triggering of cytoplasmic red fluorescent signal in HeLa cells after one-photon irradiation (λexc around 360 nm) or by the generation of a red fluorescent signal in a delineated area of a single cell after two-photon photoactivation (λexc = 770 nm).