A simple scalable method to fabricate luminescent monodisperse 200 nm europium-doped hollow TiO2 nanoshell (NS) particles is reported. Fluorophore reporter, Eup>3+p> ions are incorporated directly in the NS matrix, leaving the surface free for functionalization and the core free for payload encapsulation. Amine-functionalized polystyrene beads were used as templates, and the porous walls of europium-doped titania nanoshells were synthesized using titanium(IV) t-butoxide and europium(III) nitrate as reactants. X-ray diffraction analysis identified anatase as the predominant titania phase of the rigid nanoshell wall structure, and photoluminescence spectra showed that the Eu(III)-doped TiO2 nanoshells exhibited a red emission at 617 nm due to an atomic f鈥揻 transition. Nanoshell interactions with HeLa cervical cancer cells in vitro were visualized using two-photon microscopy of the Eu(III) emission and studied using a luminescence ratio analysis to assess nanoshell adhesion and endocytosis.