Identification of Sole (Solea solea) and Greenland Halibut (Reinhardtius hippoglossoides) by PCR Amplification of the 5S rDNA Gene

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• 作者：Ana Cé ; spedes ; Teresa Garc&iacute ; a ; Esther Carrera ; Isabel Gonzá ; lez ; Alicia Ferná ; ndez ; Pablo E. Herná ; ndez ; and Rosario Mart&iacute ; n
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1999
• 出版时间：March 1999
• 年：1999
• 卷：47
• 期：3
• 页码：1046 - 1050
• 全文大小：97K
• 年卷期：v.47,no.3(March 1999)
• ISSN：1520-5118

文摘

Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene, has been used for theidentification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides). Two species-specific primers were designed to amplify specific fragments of the 5S rDNA gene in each species.The remarkably different size of the amplicons obtained gives, by simple agarose gel electrophoresis,two distinguishable band patterns for both flatfish species. This genetic marker can be very usefulfor the accurate identification of S. solea and Greenland halibut, to enforce labeling regulations.Keywords: Species identification; Solea solea; Reinhardtius hippoglossoides; 5S rDNA; nuclearmarker