Enhanced Monoclonal Antibody Production in Hybridoma Cells by LPS and Anti-mIgG

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• 作者：Alicia Mart&iacute ; n-Ló ; pez ; Francisco Garc&iacute ; a-Camacho ; Antonio Contreras-Gó ; mez ; Emilio Molina-Grima
• 刊名：Biotechnology Progress
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：23
• 期：6
• 页码：1447 - 1453
• 全文大小：507K
• 年卷期：v.23,no.6(December 2007)
• ISSN：1520-6033

文摘

This paper reports on a methodology for increasing proliferation and monoclonal antibody (mAb)production in hybridoma cultures. The 55-6 murine B cell hybridoma line (CD40 and CD19-deficient expression) was treated with increasing concentrations of lipopolysaccharide (LPS).Expression of CD69, CD40, and CD19 surface antigens on 55-6 cells did not show significantchanges from untreated cells. The specific growth rate decreased at higher concentrations ofLPS, but the monoclonal antibody production rate was highest at the highest LPS concentrationassayed. These data are in agreement with the lowest growth rate found at this concentration ofLPS. Furthermore, cells were cultured with anti-mouse surface immunoglobulin G antibody(anti-mIgG) plus LPS to find out whether LPS-derived signals and anti-mIgG stimuli aresynergistic. CD69, CD40, and CD19 expression was greater than for either untreated cells (controlculture) or cells stimulated with LPS alone. Moreover, LPS stimulation in combination withanti-mIgG enhanced both the growth rate and IgG2a production over the control culture andcells stimulated with LPS alone. Maximum antibody concentration increased almost 500%compared to the control and about 100% with respect to culture stimulated with LPS alone. Themaximum specific IgG2a production rate was about 300% higher than in the control cultureand about 30% higher than in culture stimulated only with LPS.