Identification of the Clam Species Ruditapes decussatus (Grooved Carpet Shell), Venerupis pullastra (Pullet Carpet Shell), and Ruditapes philippinarum (Japane
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- 作者：Alicia Ferná ; ndez ; Teresa Garcí ; a ; Luis Asensio ; Miguel Á ; ngel Rodrí ; guez ; Isabel Gonzá ; lez ; Ana Cé ; spedes ; Pablo E. Herná ; ndez ; and Rosario Martí ; n
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2000
- 出版时间：August 2000
- 年：2000
- 卷：48
- 期：8
- 页码：3336 - 3341
- 全文大小：138K
- 年卷期：v.48,no.8(August 2000)
- ISSN：1520-5118
文摘
PCR-RFLP analysis has been applied to the identification of three clam species: Ruditapesdecussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), and Ruditapes philippinarum (Japanese carpet shell). PCR amplification was carried out using a set of primers designedfrom the DNA nucleotide sequences reported for 
-actins from humans and various animals.Restriction endonuclease analysis based on sequence data of the PCR products of each clam speciesrevealed the presence of species-specific polymorphic sites for MaeIII and RsaI endonucleases.Electrophoretic analysis of the amplicons digested with MaeIII and RsaI produced species-specificprofiles that allowed the genetic identification of the three clam species.Keywords: Species identification; PCR-RFLP; -actin gene; clam species; Ruditapes decussatus;Venerupis pullastra; Ruditapes philippinarum
-actins from humans and various animals.Restriction endonuclease analysis based on sequence data of the PCR products of each clam speciesrevealed the presence of species-specific polymorphic sites for MaeIII and RsaI endonucleases.Electrophoretic analysis of the amplicons digested with MaeIII and RsaI produced species-specificprofiles that allowed the genetic identification of the three clam species.Keywords: Species identification; PCR-RFLP; -actin gene; clam species; Ruditapes decussatus;Venerupis pullastra; Ruditapes philippinarum