Identification of Goose (Anser anser) and Mule Duck (Anas platyrhynchos x Cairina moschata) Foie Gras by Multiplex Polymerase Chain Reaction Amplification of the 5S RDNA Gene

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• 作者：Miguel A. Rodr&iacute ; guez ; Teresa Garc&iacute ; a ; Isabel Gonzá ; lez ; Luis Asensio ; Alicia Ferná ; ndez ; Esther Lobo ; Pablo E. Herná ; ndez ; and Rosario Mart&iacute ; n
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2001
• 出版时间：June 2001
• 年：2001
• 卷：49
• 期：6
• 页码：2717 - 2721
• 全文大小：162K
• 年卷期：v.49,no.6(June 2001)
• ISSN：1520-5118

文摘

Polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene has been used for theidentification of goose and mule duck foie gras. Two species-specific reverse primers were designedand used in a multiplex reaction, together with a forward universal primer, to amplify specificfragments of the 5S rDNA in each species. The different sizes of the species-specific amplicons,separated by agarose gel electrophoresis, allowed clear identification of goose and mule duck foiegras samples. This genetic marker can be useful for detecting fraudulent substitution of the duckliver for the more expensive goose liver.Keywords: Species identification; Anser anser; Anas platyrhynchos x Cairina moschata; 5S rDNA;foie gras