Deletion of flbA Results in Increased Secretome Complexity and Reduced Secretion Heterogeneity in Colonies of Aspergillus niger
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- 作者：Pauline Krijgsheld ; Benjamin M. Nitsche ; Harm Post ; Ana M. Levin ; Wally H. M眉ller ; Albert J. R. Heck ; Arthur F. J. Ram ; A. F. Maarten Altelaar ; Han A. B. W枚sten
- 刊名：Journal of Proteome Research
- 出版年：2013
- 出版时间：April 5, 2013
- 年：2013
- 卷：12
- 期：4
- 页码：1808-1819
- 全文大小：538K
- 年卷期：v.12,no.4(April 5, 2013)
- ISSN：1535-3907
文摘
Aspergillus niger is a cell factory for the production of enzymes. This fungus secretes proteins in the central part and at the periphery of the colony. The sporulating zone of the colony overlapped with the nonsecreting subperipheral zone, indicating that sporulation inhibits protein secretion. Indeed, strain 螖flbA that is affected early in the sporulation program secreted proteins throughout the colony. In contrast, the 螖brlA strain that initiates but not completes sporulation did not show altered spatial secretion. The secretome of 5 concentric zones of xylose-grown 螖flbA colonies was assessed by quantitative proteomics. In total 138 proteins with a signal sequence for secretion were identified in the medium of 螖flbA colonies. Of these, 18 proteins had never been reported to be part of the secretome of A. niger, while 101 proteins had previously not been identified in the culture medium of xylose-grown wild type colonies. Taken together, inactivation of flbA results in spatial changes in secretion and in a more complex secretome. The latter may be explained by the fact that strain 螖flbA has a thinner cell wall compared to the wild type, enabling efficient release of proteins. These results are of interest to improve A. niger as a cell factory.