Response Surface Methodology for Optimization of Lipase Production by an Immobilized Newly Isolated Penicillium sp.

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• 作者：Elisangela Wolski ; Elisangele Menusi ; Marcio Mazutti ; Geciane Toniazzo ; Elisandra Rigo ; Rogrio Luiz Cansian ; Altemir Mossi ; J. Vladimir Oliveira ; Marco Di Luccio ; Dbora de Oliveira ; Helen Treichel
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2008
• 出版时间：December 3, 2008
• 年：2008
• 卷：47
• 期：23
• 页码：9651-9657
• 全文大小：155K
• 年卷期：v.47,no.23(December 3, 2008)
• ISSN：1520-5045

文摘

This work reports the use of response surface methodology to optimize the lipase production by submerged fermentation using immobilized cells of a newly isolated Penicillium sp. For this, experimental designs were employed to evaluate the best condition for microorganism immobilization. The optimum experimental conditions for immobilized microorganisms were determined as 2.5% (w/v) of calcium alginate, 0.2% (w/v) of CaCl₂, and 1.5% (v/v) of glutaraldehyde and 6 h of curing time, yielding a lipase activity as high as 20.96 U/mL in 120 h of fermentation. The activity obtained is higher than those observed with free cells of this microorganism and comparable to results presented in the literature for lipase production with immobilized cells from several microorganisms. The crude enzymatic extract presented optimum pH and temperature of 7.0 and 37 °C, respectively, and maintained its activity for 60 days storage at −10 °C.