Gelatin-Based Biomaterial Engineering with Anhydride-Containing Oligomeric Cross-Linkers
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- 作者：Tina Loth ; Rudi H枚tzel ; Christian Kascholke ; Ulf Anderegg ; Michaela Schulz-Siegmund ; Michael C. Hacker
- 刊名：Biomacromolecules
- 出版年：2014
- 出版时间：June 9, 2014
- 年：2014
- 卷：15
- 期：6
- 页码：2104-2118
- 全文大小：834K
- 年卷期：v.15,no.6(June 9, 2014)
- ISSN：1526-4602
文摘
Chemically cross-linked gelatin hydrogels are versatile cell-adhesive hydrogel materials that have been established for a variety of biomedical applications. The most prominent cross-linker is glutaraldehyde, which, however, has been described to cause compatibility problems and loss of microscopic but relevant structural features. A recently developed oligomeric cross-linker that contains anhydride functionalities was evaluated as cross-linker for the fabrication of gelatin-based hydrogels and microparticles. In a fast curing reaction, hydrogels composed of gelatin and oligomeric cross-linker were fabricated with good conversion over a wide concentration range of constituents and with cross-linkers of different anhydride contents. Hydrogel properties, such as dry weight and mechanics, could be controlled by hydrogel composition and rheological properties correlated to elastic moduli from 1 to 10 kPa. The gels were shown to be cytocompatible and promoted cell adhesion. In soft formulations, cells migrated into the hydrogel bulk. Gelatin microparticles prepared by a standard water-in-oil emulsion technique were also treated with the novel oligomers, and cross-linking degrees matching those obtained with glutaraldehyde were obtained. At the same time, fewer interparticular cross-links were observed. Fluorescein-derivatized cross-linkers yielded labeled microparticles in a concentration-dependent manner. The oligomeric cross-linkers are presented as an efficient and possibly more functional and compatible alternative to glutaraldehyde. The engineered hydrogel materials hold potential for various biomedical applications.