Determination and Confirmation of the Antiviral Drug Amantadine and Its Analogues in Chicken Jerky Pet Treats

详细信息    查看全文

• 作者：Sherri B. Turnipseed ; Joseph M. Storey ; Wendy C. Andersen ; Michael S. Filigenzi ; Andrea S. Heise ; Jack J. Lohne ; Mark R. Madson ; Olgica Ceric ; Renate Reimschuessel
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 12, 2015
• 年：2015
• 卷：63
• 期：31
• 页码：6968-6978
• 全文大小：421K
• ISSN：1520-5118

文摘

In this study, we investigated two methods for the detection of antiviral compounds in chicken jerky pet treats. Initially, a screening method developed to detect many different chemical contaminants indicated the presence of amantadine, 1, in some pet treats analyzed. A second antiviral-specific method was then developed for amantadine and its analogues, rimantadine, 2, and memantine, 3. Both methods used an acidic water/acetonitrile extraction. The antiviral-specific method also included a dispersive sorbent cleanup. Analytes were detected and identified by LC-MS (ion trap and Orbitrap) instruments. The antiviral-specific method was validated by analyzing matrix blanks and fortified samples (2.5鈥?0 渭g/kg levels). Average recoveries for amantadine (using a deuterated internal standard) in fortified samples ranged from 76 to 123% with relative standard deviations of 鈮?2%. Amantadine was detected and identified in suspect chicken jerky pet treat samples at levels ranging from <2.5 渭g/kg to over 600 渭g/kg. Rimantadine and memantine were not detected in any samples.