Characterization of Potato Leaf Starch
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- 作者：Stalin Santacruz ; Kristine Koch ; Roger Andersson ; and Per Å ; man
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：April 7, 2004
- 年：2004
- 卷：52
- 期：7
- 页码：1985 - 1989
- 全文大小：150K
- 年卷期：v.52,no.7(April 7, 2004)
- ISSN：1520-5118
文摘
The starch accumulation-degradation process as well as the structure of leaf starch are not completelyunderstood. To study this, starch was isolated from potato leaves collected in the early morning andlate afternoon in July and August, representing different starch accumulation rates. The starch contentof potato leaves varied between 2.9 and 12.9% (dry matter basis) over the night and day in themiddle of July and between 0.6 and 1.5% in August. Scanning electron microscopy analyses of thefour isolated starch samples showed that the granules had either an oval or a round shape and didnot exceed 5 
m in size. Starch was extracted by successive washing steps with dimethyl sulfoxideand precipitated with ethanol. An elution profile on Sepharose CL-6B of debranched starch showedthe presence of a material with a chain length distribution between that generally found for amyloseand amylopectin. Amylopectin unit chains of low molecular size were present in a higher amount inthe afternoon than in the morning samples. What remains at the end of the night is depleted in specificchain lengths, mainly between DP 15 and 24 and above DP 35, relative to the end of the day.Keywords: Leaf starch; potato starch; starch extraction; starch accumulation; DMSO
m in size. Starch was extracted by successive washing steps with dimethyl sulfoxideand precipitated with ethanol. An elution profile on Sepharose CL-6B of debranched starch showedthe presence of a material with a chain length distribution between that generally found for amyloseand amylopectin. Amylopectin unit chains of low molecular size were present in a higher amount inthe afternoon than in the morning samples. What remains at the end of the night is depleted in specificchain lengths, mainly between DP 15 and 24 and above DP 35, relative to the end of the day.Keywords: Leaf starch; potato starch; starch extraction; starch accumulation; DMSO