Using Bicellar Mixtures To Form Supported and Suspended Lipid Bilayers on Silicon Chips

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• 作者：Reema Zeineldin ; Julie A. Last ; Andrea L. Slade ; Linnea K. Ista ; Paul Bisong ; Michael J. O'Brien ; S. R. J. Brueck ; Darryl Y. Sasaki ; Gabriel P. Lopez
• 刊名：Langmuir
• 出版年：2006
• 出版时间：September 12, 2006
• 年：2006
• 卷：22
• 期：19
• 页码：8163 - 8168
• 全文大小：739K
• 年卷期：v.22,no.19(September 12, 2006)
• ISSN：1520-5827

文摘

Bicellar mixtures, planar lipid bilayer assemblies comprising long- and short-chain phosphatidylcholine lipids insuspension, were used to form supported lipid bilayers on flat silicon substrate and on nanotextured silicon substratescontaining arrays of parallel troughs (170 nm wide, 380 nm deep, and 300 nm apart). Confocal fluorescence and atomicforce microscopies were used to characterize the resulting lipid bilayer. Formation of a continuous biphasic undulatinglipid bilayer membrane, where the crests and troughs corresponded to supported and suspended lipid bilayer regions,is demonstrated. The use of interferometric lithography to fabricate nanotexured substrates provides an advantage overother nanotextured substrates such as nanoporous alumina by offering flexibility in designing different geometriesfor suspending lipid bilayers.