Thiamine Pyrophosphate Stimulates Acetone Activation by Desulfococcus biacutus As Monitored by a Fluorogenic ATP Analogue

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• 作者：Olga B. Guti茅rrez Acosta ; Norman Hardt ; Stephan M. Hacker ; Tobias Strittmatter ; Bernhard Schink ; Andreas Marx
• 刊名：ACS Chemical Biology
• 出版年：2014
• 出版时间：June 20, 2014
• 年：2014
• 卷：9
• 期：6
• 页码：1263-1266
• 全文大小：253K
• 年卷期：v.9,no.6(June 20, 2014)
• ISSN：1554-8937

文摘

Acetone can be degraded by aerobic and anaerobic microorganisms. Studies with the strictly anaerobic sulfate-reducing bacterium Desulfococcus biacutus indicate that acetone degradation by these bacteria starts with an ATP-dependent carbonylation reaction leading to acetoacetaldehyde as the first reaction product. The reaction represents the second example of a carbonylation reaction in the biochemistry of strictly anaerobic bacteria, but the exact mechanism and dependence on cofactors are still unclear. Here, we use a novel fluorogenic ATP analogue to investigate its mechanism. We find that thiamine pyrophosphate is a cofactor of this ATP-dependent reaction. The products of ATP cleavage are AMP and pyrophosphate, providing first insights into the reaction mechanism by indicating that the reaction proceeds without intermediate formation of acetone enol phosphate.