Snapshot of a DNA Polymerase while Incorporating Two Consecutive C5-Modified Nucleotides

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• 作者：Samra Obeid ; Holger Bu脽kamp ; Wolfram Welte ; Kay Diederichs ; Andreas Marx
• 刊名：Journal of the American Chemical Society
• 出版年：2013
• 出版时间：October 23, 2013
• 年：2013
• 卷：135
• 期：42
• 页码：15667-15669
• 全文大小：257K
• 年卷期：v.135,no.42(October 23, 2013)
• ISSN：1520-5126

文摘

Functional nucleotides are important in many cutting-edge biomolecular techniques. Often several modified nucleotides have to be incorporated consecutively. This structural study of KlenTaq DNA polymerase, a truncated form of Thermus aquaticus DNA polymerase, gives first insights how multiple modifications are processed by a DNA polymerase and, therefore, contribute to the understanding of these enzymes in their interplay with artificial substrates.