Biomimetic Calcium Phosphate Mineralization with Multifunctional Elastin-Like Recombinamers

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• 作者：Susana Prieto ; Andriy Shkilnyy ; Claudia Rumplasch ; Artur Ribeiro ; F. Javier Arias ; J. Carlos Rodri虂guez-Cabello ; Andreas Taubert
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：May 9, 2011
• 年：2011
• 卷：12
• 期：5
• 页码：1480-1486
• 全文大小：946K
• 年卷期：v.12,no.5(May 9, 2011)
• ISSN：1526-4602

文摘

Biomimetic hybrid materials based on a polymeric and an inorganic component such as calcium phosphate are potentially useful for bone repair. The current study reports on a new approach toward biomimetic hybrid materials using a set of recombinamers (recombinant protein materials obtained from a synthetic gene) as crystallization additive for calcium phosphate. The recombinamers contain elements from elastin, an elastic structural protein, and statherin, a salivary protein. Via genetic engineering, the basic elastin sequence was modified with the SN_A15 domain of statherin, whose interaction with calcium phosphate is well-established. These new materials retain the biocompatibility, 鈥渟mart鈥?nature, and desired mechanical behavior of the elastin-like recombinamer (ELR) family. Mineralization in simulated body fluid (SBF) in the presence of these recombinamers reveals surprising differences. Two of the polymers inhibit calcium phosphate deposition (although they contain the statherin segment). In contrast, the third polymer, which has a triblock structure, efficiently controls the calcium phosphate formation, yielding spherical hydroxyapatite (HAP) nanoparticles with diameters from 1 to 3 nm after 1 week in SBF at 37 掳C. However, at lower temperatures, no precipitation is observed with any of the polymers. The data thus suggest that the molecular design of ELRs containing statherin segments and the selection of an appropriate polymer structure are key parameters to obtain functional materials for the development of intelligent systems for hard tissue engineering and subsequent in vivo applications.