Determination of Furaltadone and Nifursol Residues in Poultry Eggs by Liquid Chromatography鈥揈lectrospray Ionization Tandem Mass Spectrometry
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- 作者：Jorge Barbosa ; Andreia Freitas ; Jos茅 Luis Mour茫o ; Maria Irene Noronha da Silveira ; Fernando Ramos
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：May 2, 2012
- 年：2012
- 卷：60
- 期：17
- 页码：4227-4234
- 全文大小：276K
- 年卷期：v.60,no.17(May 2, 2012)
- ISSN：1520-5118
文摘
The use of nitrofurans as veterinary drugs has been banned from intensive animal production in the European Union (EU) since 1993. The objective of the present study was to evaluate the accumulation and depletion of furaltadone and nifursol and their side-chain metabolites 5-methylmorpholino-3-amino-2-oxazolidinone (AMOZ) and 3,5-dinitrosalicylic acid hydrazide (DNSAH) in eggs after administration of therapeutic and subtherapeutic doses of the drugs to laying hens during three consecutive weeks. LC-MS/MS, with positive and negative electrospray ionization methods, was used for the determination of parent compounds and metabolites in yolk and egg white and was validated according to criteria established by Commission Decision 2002/657/EC. The decision limit (CC伪) and the detection capability (CC尾) of the analytical methodology for metabolites were 0.1 and 0.5 渭g/kg for AMOZ and 0.3 and 0.9 渭g/kg for DNSAH, respectively. For the parent compounds, CC伪 and CC尾 were 0.9 and 2.0 渭g/kg for furaltadone and 1.3 and 3.1 渭g/kg for nifursol, respectively. The data obtained show that the parent compounds are much less persistent than their side-chain metabolites in either yolk or egg white. Between the studied metabolites, AMOZ is the most persistent and could be detected in either yolk or egg white three weeks following withdrawal from treatment.