X-ray Spectromicroscopy Study of Protein Adsorption to a Polystyrene−Polylactide Blend

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• 作者：Bonnie O. Leung ; Adam P. Hitchcock ; Rena Cornelius ; John L. Brash ; Andreas Scholl ; Andrew Doran
• 刊名：Biomacromolecules
• 出版年：2009
• 出版时间：July 13, 2009
• 年：2009
• 卷：10
• 期：7
• 页码：1838-1845
• 全文大小：496K
• 年卷期：v.10,no.7(July 13, 2009)
• ISSN：1526-4602

文摘

Synchrotron-based X-ray photoemission electron microscopy (X-PEEM) was used to study the adsorption of human serum albumin (HSA) to polystyrene-polylactide (40:60 PS-PLA, 0.7 wt %) thin films, annealed under various conditions. The rugosity of the substrate varied from 35 to 90 nm, depending on the annealing conditions. However, the characteristics of the protein adsorption (amounts and phase preference) were not affected by the changes in topography. The adsorption was also not changed by the phase inversion which occured when the PS-PLA substrate was annealed above T_g of the PLA. The amount of protein adsorbed depended on whether adsorption took place from distilled water or phosphate buffered saline solution. These differences are interpreted as a result of ionic strength induced changes in the protein conformation in solution.