Composite Magnetite and Protein Containing CaCO3 Crystals. External Manipulation and Vaterite 鈫?Calcite Recrystallization-Mediated Release Performance

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• 作者：Alena Sergeeva ; Roman Sergeev ; Ekaterina Lengert ; Andrey Zakharevich ; Bogdan Parakhonskiy ; Dmitry Gorin ; Sergey Sergeev ; Dmitry Volodkin
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：September 30, 2015
• 年：2015
• 卷：7
• 期：38
• 页码：21315-21325
• 全文大小：512K
• ISSN：1944-8252

文摘

Biocompatibility and high loading capacity of mesoporous CaCO₃ vaterite crystals give an option to utilize the polycrystals for a wide range of (bio)applications. Formation and transformations of calcium carbonate polymorphs have been studied for decades, aimed at both basic and applied research interests. Here, composite multilayer-coated calcium carbonate polycrystals containing Fe₃O₄ magnetite nanoparticles and model protein lysozyme are fabricated. The structure of the composite polycrystals and vaterite 鈫?calcite recrystallization kinetics are studied. The recrystallization results in release of both loaded protein and Fe₃O₄ nanoparticles (magnetic manipulation is thus lost). Fe₃O₄ nanoparticles enhance the recrystallization that can be induced by reduction of the local pH with citric acid and reduction of the polycrystal crystallinity. Oppositely, the layer-by-layer assembled poly(allylamine hydrochloride)/poly(sodium styrenesulfonate) polyelectrolyte coating significantly inhibits the vaterite 鈫?calcite recrystallization (from hours to days) most likely due to suppression of the ion exchange giving an option to easily tune the release kinetics for a wide time scale, for example, for prolonged release. Moreover, the recrystallization of the coated crystals results in formulation of multilayer capsules keeping the feature of external manipulation. This study can help to design multifunctional microstructures with tailor-made characteristics for loading and controlled release as well as for external manipulation.