Fluorescent Signal Amplification of Carbocyanine Dyes Using Engineered Viral Nanoparticles

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• 作者：Carissa M. Soto ; Amy Szuchmacher Blum ; Gary J. Vora ; Nikolai Lebedev ; Carolyn E. Meador ; Angela P. Won ; Anju Chatterji ; John E. Johnson ; and Banahalli R. Ratna
• 刊名：Journal of the American Chemical Society
• 出版年：2006
• 出版时间：April 19, 2006
• 年：2006
• 卷：128
• 期：15
• 页码：5184 - 5189
• 全文大小：400K
• 年卷期：v.128,no.15(April 19, 2006)
• ISSN：1520-5126

文摘

We report enhancement in the fluorescent signal of the carbocyanine dye Cy5 by using anengineered virus as a scaffold to attach >40 Cy5 reporter molecules at fixed locations on the viral capsid.Although cyanine dye loading is often accompanied by fluorescence quenching, our results demonstratethat organized spatial distribution of Cy5 reporter molecules on the capsid obviates this commonlyencountered problem. In addition, we observe energy transfer from the virus to adducted dye molecules,resulting in a highly fluorescent viral nanoparticle. We have used this enhanced fluorescence for the detectionof DNA-DNA hybridization. When compared with the most often used detection methods in a microarray-based genotyping assay for Vibrio cholerae O139, these viral nanoparticles markedly increased assaysensitivity, thus demonstrating their applicability for existing DNA microarray protocols.