Threshold Detection Using Indicator-Displacement Assays: An Application in the Analysis of Malate in Pinot Noir Grapes
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- 作者：Anna M. Pi
//pubs.acs.org/images/entities/acedil.gif" border="0">tek ; Yannick J. Bomble ; Sheryl L. Wiskur ; and Eric V. Anslyn
- 刊名：Journal of the American Chemical Society
- 出版年：2004
- 出版时间：May 19, 2004
- 年：2004
- 卷：126
- 期：19
- 页码：6072 - 6077
- 全文大小：179K
- 年卷期：v.126,no.19(May 19, 2004)
- ISSN：1520-5126
文摘
The mathematics for modeling indicator-displacement assay isotherms is presented andcontrasted to the classical host-guest binding isotherm. It is shown that the signal response can be tunedto occur closer to 1 equiv of guest relative to a standard binding algorithm. This delay in response leadsto a better triggering protocol for threshold detection schemes. The determination of malate in Pinot Noirmust was calculated using this new mathematical model, which demonstrates how a color change can betuned to occur near a desired concentration of analyte.