Molecularly Resolved Images of Peptide-Functionalized Gold Surfaces by Scanning Tunneling Microscopy

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• 作者：Annette F. Raigoza ; Lauren J. Webb
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：November 28, 2012
• 年：2012
• 卷：134
• 期：47
• 页码：19354-19357
• 全文大小：251K
• 年卷期：v.134,no.47(November 28, 2012)
• ISSN：1520-5126

文摘

Peptide-terminated monolayers were formed through a Huisgen cycloaddition reaction between an 伪-helical peptide containing two propargylglycine unnatural functional groups 20 脜 apart and an alkanethiol self-assembled monolayer (SAM) on a gold surface containing 25% surface density of reactive azide terminal groups. The azide- and peptide-terminated surfaces were imaged by scanning tunneling microscopy (STM) using a low tunneling current of 10 pA. On the peptide-terminated surface, oblong features 30 脜 long and 20 脜 wide were observed and attributed to individual surface-bound 伪-helical peptides oriented parallel to the gold surface. These features covered an area of the surface corresponding to a density of 0.11 卤 0.01 peptides nm^鈥?, compared with a theoretical density of 0.14 peptides nm^鈥? for a fully reacted surface. Finally, no evidence of peptide aggregation was observed on either short (<10 nm) or long (100 nm) length scales.