Gold Nanocluster-Assisted Fluorescent Detection for Hydrogen Peroxide and Cholesterol Based on the Inner Filter Effect of Gold Nanoparticles

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• 作者：Heng-Chia Chang ; Ja-an Annie Ho
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：October 20, 2015
• 年：2015
• 卷：87
• 期：20
• 页码：10362-10367
• 全文大小：374K
• ISSN：1520-6882

文摘

We developed a simple, sensitive inner filter effect (IFE)-based fluorescent assay for sensing H₂O₂ and cholesterol. In the process, poly(vinylpyrrolidone)-protected gold nanoparticles (PVP-AuNPs) and fluorescent BSA-protected gold nanoclusters (BSA-AuNCs) were used as an IFE absorber/fluorophore pair. PVP-AuNPs can be a powerful absorber to influence the emission of the fluorophore, BSA-AuNCs, in the IFE-based fluorescent assays. That is due to the high extinction coefficient of AuNPs and the complementary overlap between the surface plasmon resonance (SPR) absorption of PVP-AuNPs and the excitation of BSA-AuNCs. The PVP-Au seeds, produced by directly mixing PVP with HAuCl₄, were able to catalyze H₂O₂ to enlarge AuNPs. The SPR absorption of PVP-AuNPs was enhanced with an increased concentration of H₂O₂ and, subsequently, induced significant fluorescence quenching of BSA-AuNCs. The IFE-based fluorescent assay enabled the detection of H₂O₂ and generation of H₂O₂ in the presence of O₂/cholesterol and cholesterol oxidase (ChOx) by the fluorescence response of BSA-AuNCs. The present IFE-based approach can detect H₂O₂ ranging from 1 to 100 渭M with a detection limit of 0.8 渭M and cholesterol ranging from 1 to 100 渭M with a detection limit of 1.4 渭M.