An Automated Noncontact Deposition Interface for Liquid Chromatography Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
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- 作者：Christer Ericson ; Qui T. Phung ; David M. Horn ; Eric C. Peters ; Jonathan R. Fitchett ; Scott B. Ficarro ; Arthur R. Salomon ; Laurence M. Brill ; and Ansgar Brock
- 刊名：Analytical Chemistry
- 出版年：2003
- 出版时间：May 15, 2003
- 年：2003
- 卷：75
- 期：10
- 页码：2309 - 2315
- 全文大小：515K
- 年卷期：v.75,no.10(May 15, 2003)
- ISSN：1520-6882
文摘
A new multichannel deposition system was developed foroff-line liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry (LC/MALDI-MS).This system employs a pulsed electric field to transfer theeluents from multiple parallel columns directly ontoMALDI targets without the column outlets touching thetarget surface. The deposition device performs well witha wide variety of solvents that have different viscosities,vapor pressures, polarities, and ionic strengths. Surface-modified targets were used to facilitate concentration andprecise positioning of samples, allowing for efficientautomation of high-throughput MALDI analysis. Theoperational properties of this system allow the user toprepare samples using MALDI matrixes whose propertiesrange from hydrophilic to hydrophobic. The latter, exemplified by 
-cyano-4-hydroxycinnamic acid, were typicallyprocessed with a multistep deposition method consistingof precoating of individual spots on the target plate,sample deposition, and sample recrystallization steps.Using this method, 50 amol of angiotensin II was detectedreproducibly with high signal-to-noise ratio after LCseparation. Experimental results show that there is nosignificant decrease in chromatographic resolution usingthis device. To assess the behavior of the apparatus forcomplex mixtures, 5 
g of a tryptic digest of the cytosolicproteins of yeast was analyzed by LC/MALDI-MS andmore than 13 500 unique analytes were detected in asingle LC/MS analysis.
-cyano-4-hydroxycinnamic acid, were typicallyprocessed with a multistep deposition method consistingof precoating of individual spots on the target plate,sample deposition, and sample recrystallization steps.Using this method, 50 amol of angiotensin II was detectedreproducibly with high signal-to-noise ratio after LCseparation. Experimental results show that there is nosignificant decrease in chromatographic resolution usingthis device. To assess the behavior of the apparatus forcomplex mixtures, 5 g of a tryptic digest of the cytosolicproteins of yeast was analyzed by LC/MALDI-MS andmore than 13 500 unique analytes were detected in asingle LC/MS analysis.