Permeability Effects on the Efficiency of Antioxidant Nanoreactors

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• 作者：Iria Louzao ; Jan C. M. van Hest
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：July 8, 2013
• 年：2013
• 卷：14
• 期：7
• 页码：2364-2372
• 全文大小：513K
• 年卷期：v.14,no.7(July 8, 2013)
• ISSN：1526-4602

文摘

Enzyme-loaded polymeric vesicles, or polymersomes, can be regarded as nanoreactors, which, for example, can be applied as artificial organelles. We implemented a naturally occurring enzymatic cascade reaction in two types of polymersomes, which are known to possess different permeability properties. The selected cascade reaction involved the antioxidant enzymes superoxide dismutase (SOD1) and catalase (CAT) for combating oxidative stress. The activities of both enzymes were investigated by spectrophotometric and electrochemical assays. Whereas the SOD1 substrate (the radical anion superoxide, O₂^{鈥⑩€?/sup>) was able to penetrate both membranes equally well, the CAT substrate (H₂O₂) showed different rates of diffusion. When O₂鈥⑩€?/sup> was generated inside polymersomes filled with both SOD1 and CAT, the activities of the two systems were comparable again.}