Development and Characterization of New Cyclodextrin Polymer-Based DNA Delivery Systems
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- 作者：Virginie Burckbuchler ; Vronique Wintgens ; Christian Leborgne ; Sophie Lecomte ; Nadine Leygue ; Daniel Scherman ; Antoine Kichler ; Catherine Amiel
- 刊名：Bioconjugate Chemistry
- 出版年：2008
- 出版时间：December 17, 2008
- 年：2008
- 卷：19
- 期：12
- 页码：2311-2320
- 全文大小：399K
- 年卷期：v.19,no.12(December 17, 2008)
- ISSN：1520-4812
文摘
In this study, we investigated whether a cyclodextrin polymer (polyβCD) complexed with cationic adamantyl derivatives (Ada) could be used as a vector for gene delivery. DNA compaction as a function of adamantyl/DNA phosphate ratio (A/P) by this new class of vector was demonstrated using surface enhanced Raman spectroscopy, ζ potential measurements, and DNA retardation assays. Transfection data highlight the relationship between in vitro gene delivery efficiency and the combination of several physical properties of the polyβCD/Ada/DNA polyplexes, including cationic polar headgroup valency and chemical structure of the spacer arm of Ada connectors, the adamantyl/DNA phosphate ratio (A/P) of the polyβCD/Ada/DNA polyplexes, and the ionic strength of the medium. Finally, when associating the best formulation with a fusogenic peptide, we reached transfection levels which were of the same order as those obtained with DOTAP.