Versatile Lock and Key Assembly for Optical Measurements with Microfluidic Platforms and Cartridges

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• 作者：Oriol Ymbern ; Miguel Berenguel-Alonso ; Antonio Calvo-L贸pez ; Sara G贸mez-de Pedro ; David Izquierdo ; Juli谩n Alonso-Chamarro
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：February 3, 2015
• 年：2015
• 卷：87
• 期：3
• 页码：1503-1508
• 全文大小：314K
• ISSN：1520-6882

文摘

A novel and versatile optical reader for microfluidic platforms is presented. The reader includes a modular insertion port based on the lock and key concept for reproducible alignment with a miniaturized optical detection system comprising an interchangeable light emitting diode (LED) and a photodiode. The modular nature of the insertion port allows the use of microfluidic platforms in variable shapes and fluidic configurations. Three different analytical methodologies based on absorbance or fluorescence measurements were used to demonstrate the flexibility and reproducibility of the proposed experimental setup.