Determination of the Major Phenolic Compounds in Pomegranate Juices by HPLC鈥揇AD鈥揈SI-MS
详细信息 查看全文
- 作者：Ana Mar铆a G贸mez-Caravaca ; Vito Verardo ; Moreno Toselli ; Antonio Segura-Carretero ; Alberto Fern谩ndez-Guti茅rrez ; Maria Fiorenza Caboni
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：June 5, 2013
- 年：2013
- 卷：61
- 期：22
- 页码：5328-5337
- 全文大小：302K
- 年卷期：v.61,no.22(June 5, 2013)
- ISSN：1520-5118
文摘
Traditionally, pomegranate (Punica granatum L.) has been consumed as fresh fruit or as pomegranate juice. In this study, the main phenolic compounds of 12 pomegranate varieties and 5 pomegranate clones were determined by HPLC鈥揇AD鈥揈SI-MS. Two chromatographic methods with a fused-core C18 column and a classical HPLC system were developed. Thirteen anthocyanins and fourteen other phenolic compounds were determined in the pomegranate juices. As far as we are concerned, a new flavonol-glycoside, phellatin or its isomer amurensin, has been tentatively identified for the first time in pomegranate juices. Total phenolic content ranged from 580.8 to 2551.3 mg/L of pomegranate juice. Anthocyanins varied between 20 to 82% of total phenolic content. Flavonoids were 1.6鈥?3.6% of total phenolic compounds, while phenolic acids and ellagitannins were in the range 16.4鈥?5.8%. The five clones reported a phenolic content comparable with that of the other pomegranate samples.