Enhanced Interfacial Adhesion and Osteogenesis for Rapid 鈥淏one-like鈥?Biomineralization by PECVD-Based Silicon Oxynitride Overlays

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• 作者：Azhar Ilyas ; Nickolay V. Lavrik ; Harry K. W. Kim ; Pranesh B. Aswath ; Venu G. Varanasi
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：July 22, 2015
• 年：2015
• 卷：7
• 期：28
• 页码：15368-15379
• 全文大小：608K
• ISSN：1944-8252

文摘

Structurally unstable fracture sites require metal fixative devices, which have long healing times due to their lack of osteoinductivity. Bioactive glass coatings lack in interfacial bonding, delaminate, and have reduced bioactivity due to the high temperatures used for their fabrication. Here, we test the hypothesis that low-temperature PECVD amorphous silica can enhance adhesion to the underlying metal surface and that N incorporation enhances osteogenesis and rapid biomineralization. A model Ti/TiO₂鈥揝iO_x interface was formed by first depositing Ti onto Si wafers, followed by surface patterning, thermal annealing to form TiO₂, and depositing SiO_x/Si(ON)_x overlays. TEM micrographs showed conformal SiO_x layers on Ti/TiO₂ overlays while XPS data revealed the formation of an elemental Ti鈥揙鈥揝i interface. Nanoscratch testing verified strong SiO_x bonding with the underlying TiO₂ layers. In vitro studies showed that the surface properties changed significantly to reveal the formation of hydroxycarbonate apatite within 6 h, and Si(ON)_x surface chemistry induced osteogenic gene expression of human periosteal cells and led to a rapid 鈥渂one-like鈥?biomineral formation within 4 weeks. XANES data revealed that the incorporation of N increased the surface HA bioactivity by increasing the carbonate to phosphate ratio. In conclusion, silicon oxynitride overlays on bone-implant systems enhance osteogenesis and biomineralization via surface nitrogen incorporation.