文摘
Functionalization of electrospun mats with antimicrobial nanomaterials is an attractive strategy to develop polymer coating materials to prevent bacterial colonization on surfaces. In this study we demonstrated a feasible approach to produce antimicrobial electrospun mats through a postfabrication binding of graphene-based nanocomposites to the nanofibers鈥?surface. A mixture of poly(lactide-co-glycolide) (PLGA) and chitosan was electrospun to yield cylindrical and narrow-diameter (356 nm) polymeric fibers. To achieve a robust antimicrobial property, the PLGA鈥揷hitosan mats were functionalized with graphene oxide decorated with silver nanoparticles (GO鈥揂g) via a chemical reaction between the carboxyl groups of graphene and the primary amine functional groups on the PLGA鈥揷hitosan fibers using 3-(dimethylamino)propyl-N鈥?ethylcarbodiimide hydrochloride and N-hydroxysuccinimide as cross-linking agents. The attachment of GO鈥揂g sheets to the surface of PLGA鈥揷hitosan fibers was successfully revealed by scanning and transmission electron images. Upon direct contact with bacterial cells, the PLGA鈥揷hitosan mats functionalized with GO鈥揂g nanocomposites were able to effectively inactivate both Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria. Our results suggest that covalent binding of GO鈥揂g nanocomposites to the surface of PLGA鈥揷hitosan mats opens up new opportunities for the production of cost-effective, scalable, and biodegradable coating materials with the ability to hinder microbial proliferation on solid surfaces.