Submicrometer Plate Heights for Capillaries Packed with Silica Colloidal Crystals

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• 作者：Douglas S. Malkin ; Bingchuan Wei ; Arthur J. Fogiel ; Sau Lan Staats ; Mary J. Wirth
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：March 15, 2010
• 年：2010
• 卷：82
• 期：6
• 页码：2175-2177
• 全文大小：141K
• 年卷期：v.82,no.6(March 15, 2010)
• ISSN：1520-6882

文摘

Extremely uniform packing of colloidal silica in capillaries is shown. Reversed-phase electrochromatograms of DiI-C₁₂ exhibit plate heights as low as 0.23 μm and a reduced plate height as low as 0.7, using 75 μm i.d. capillaries packed with 330 nm silica particles. The contribution from the A term is 0 ± 20 nm in electrochromatography. The particles are shown to form colloidal crystals inside the capillaries. Optical images show Bragg diffraction, indicative of crystallinity. Scanning electron microscopy (SEM) images show face-centered cubic crystallinity, and the porosity is 0.25 ± 0.01, which is in agreement with that for face-centered cubic crystals. The capillaries are fritless, and 100 μm i.d. capillaries packed with silica colloidal crystals withstand pressures of at least 12400 psi.