Enzyme-Based NAND and NOR Logic Gates with Modular Design

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• 作者：Jian Zhou ; Mary A. Arugula ; Jan Halmek ; Marcos Pita ; Evgeny Katz
• 刊名：Journal of Physical Chemistry B
• 出版年：2009
• 出版时间：December 10, 2009
• 年：2009
• 卷：113
• 期：49
• 页码：16065-16070
• 全文大小：259K
• 年卷期：v.113,no.49(December 10, 2009)
• ISSN：1520-5207

文摘

The logic gates NAND/NOR were mimicked by enzyme biocatalyzed reactions activated by sucrose, maltose and phosphate. The subunits performing AND/OR Boolean logic operations were designed using maltose phosphorylase and cooperative work of invertase/amyloglucosidase, respectively. Glucose produced as the output signal from the AND/OR subunits was applied as the input signal for the INVERTER gate composed of alcohol dehydrogenase, glucose oxidase, microperoxidase-11, ethanol and NAD⁺, which generated the final output in the form of NADH inverting the logic signal from 0 to 1 or from 1 to 0. The final output signal was amplified by a self-promoting biocatalytic system. In order to fulfill the Boolean properties of associativity and commutativity in logic networks, the final NADH output signal was converted to the initial signals of maltose and phosphate, thus allowing assembling of the same standard units in concatenated sequences. The designed modular approach, signal amplification and conversion processes open the way toward complex logic networks composed of standard elements resembling electronic integrated circuitries.