Influence of Soy Aglycon Isoflavones on Bone-Related Traits and Lens Protein Characteristics of Ovariectomized Rats and Bioactivity Performance of Osteoprogenitor Cells
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- 作者：Tu-Fa Lien ; Wenlung Chen ; Yu-Lin Hsu ; Hsiu-Ling Chen ; Robin Y.-Y. Chiou
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：October 18, 2006
- 年：2006
- 卷：54
- 期：21
- 页码：8027 - 8032
- 全文大小：71K
- 年卷期：v.54,no.21(October 18, 2006)
- ISSN：1520-5118
文摘
Health benefits of soy isoflavones have attracted the concern of the public and the interest of health-care professionals. In this study, two trials were conducted in characterizing bone-related traits andlens proteins as affected by supplementation of soy aglycon isoflavones (SAI). In trial 1, an in vivostudy, 20 Sprague-Dawley rats were ovariectomized (OVX) and randomly distributed into OVX andOVX+SAI (135 mg of SAI/kg of feed; 8.33 mg/kg body weight; 2.5 mg/day) groups. Another groupcontaining 10 rats with a sham operation was control (Sham). The experiment period was 3 months,and the rats were subjected to bone-related traits and lens protein characterization. In trial 2, an invitro study, osteoprogenitor cells (UMR-106) were divided into SAI-supplemented (0.5 mg of SAI/mLof medium) and unsupplemented groups. Results of the in vivo study indicated that daily BW gainsin the OVX and OVX+SAI groups were greater than that of the Sham group (p < 0.05). Bone ashand Ca contents of the Sham and OVX+SAI groups were higher than those of the OVX group (p <0.05), while bone density, strength, and phosphorus contents among groups varied insignificantly (p> 0.05). When the lens proteins were extracted and analyzed with size-exclusion HPLC, the contentsof 
es/gifchars/beta2.gif" BORDER=0 ALIGN="middle">- and es/gifchars/gamma.gif" BORDER=0 >-crystallins were lowest in the OVX group and the protein solubility decrease could berecovered by dietary SAI supplementation (shown by OVX+SAI group). Based on Raman spectra ofthe isolated lens proteins, disulfide bonds were observed more in OVX lens than in the Sham andOVX+SAI lens. Results of in vitro study with osteoprogenitor cells revealed that cell viability, alkalinephosphatase activity, osteocalcin, and Ca contents of the SAI-supplemented group were higher thanthose of the unsupplemented group (p < 0.05). The likely potency to enhance bone and lens healthby SAI supplementation is worth pointing out.