Enhanced Long-Term Stability for Single Ion Channel Recordings Using Suspended Poly(lipid) Bilayers
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- 作者：Benjamin A. Heitz ; Juhua Xu ; Henry K. Hall ; ; Craig A. Aspinwall ; S. Scott Saavedra
- 刊名：Journal of the American Chemical Society
- 出版年：2009
- 出版时间：May 20, 2009
- 年：2009
- 卷：131
- 期：19
- 页码：6662-6663
- 全文大小：130K
- 年卷期：v.131,no.19(May 20, 2009)
- ISSN：1520-5126
文摘
Black lipid membranes (BLMs) are widely used for recording the activity of incorporated ion channel proteins. However, BLMs are inherently unstable structures that typically rupture within a few hours after formation. Here, stabilized BLMs were formed using the polymerizable lipid bis-dienoyl phosphatidylcholine (bis-DenPC) on glass pipettes of 10 μm (I.D.). After polymerization, these BLMs maintained steady conductance values for several weeks, as compared to a few hours for unpolymerized membranes. The activity of an ion channel, α-hemolysin, incorporated into bis-DenPC BLMs prior to polymerization, was maintained for 1 week after BLM formation and polymerization. These lifetimes are a substantial improvement over those achievable with conventional BLM technologies. Polymerized BLMs containing functional ion channels may represent an enabling technology for development of robust biosensors and drug screening devices.