Multiparametric Quantitation of the Bacillus cereus Toxins Cereulide and Isocereulides A鈥揋 in Foods

详细信息    查看全文

• 作者：Sandra Marxen ; Timo D. Stark ; Andrea R眉tschle ; Genia L眉cking ; Elrike Frenzel ; Siegfried Scherer ; Monika Ehling-Schulz ; Thomas Hofmann
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 23, 2015
• 年：2015
• 卷：63
• 期：37
• 页码：8307-8313
• 全文大小：306K
• ISSN：1520-5118

文摘

Consumption of food products contaminated with cereulide (1), a toxin produced by Bacillus cereus, might cause intoxications with symptoms reported to range from indigestion pain and emesis to death. Recently, a series of structural variants, coined isocereulides A鈥揋 (2鈥?b>8), were identified for the first time to be produced along with cereulide (1). The observation that isocereulide A (2) shows an 鈭?-fold increased cytotoxicity when compared to 1 urges the development of analytical tools enabling an accurate quantitation of these toxins. Therefore, a rapid, sensitive, and robust stable isotope dilution assay (SIDA) was developed for the combined quantitation of 1鈥?b>8 by means of UPLC-MS/MS. On average, trueness and precision of the method were 112.5 卤 1.8% RSD, repeatability and reproducibility were 2 and 4% for cereulide and isocereulides A鈥揋, and the LOD and LOQ of 0.1 and 0.5 ng/g, respectively, demonstrated a high sensitivity for the developed SIDA method. Application of this method to food samples revealed elevated levels of 1鈥?b>8 in two suspicious noodle samples, for example, ranging from 0.59 (7) to 189.08 ng/g (1) in sample 1 and from 5.77 (7) to 6198.17 ng/g (1) in sample 2, whereas the analysis of 25 randomly selected food samples, which have not been the subject to any complaints, did not contain detectable amounts of any of these toxins. As a consequence, this SIDA method could add an important contribution to the knowledge-based risk assessment of B. cereus toxins in foods.