Self-Assembled Supramolecular Nanoprobes for Ratiometric Fluorescence Measurement of Intracellular pH Values

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• 作者：Leiliang He ; Xiaohai Yang ; Fang Zhao ; Kemin Wang ; Qing Wang ; Jianbo Liu ; Jin Huang ; Wenshan Li ; Meng Yang
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：February 17, 2015
• 年：2015
• 卷：87
• 期：4
• 页码：2459-2465
• 全文大小：371K
• ISSN：1520-6882

文摘

Self-assembly of small building blocks into functional supramolecular nanostructure has opened prospects for the design of novel materials. With this molecular engineering strategy, we have developed self-assembled supramolecular nanoprobes (SSNPs) for ratiometric fluorescence measurement of pH values in cells. The nanoprobes with a diameter of 鈭?0 nm could be formulated just by mixing pH-sensitive adamantane鈥揻luorescein (Ad-F) and pH-insensitive adamantane鈥揜hodamine B (Ad-R) with 尾-cyclodextrin polymer (poly-尾-CD) at one time. The nanoprobes with good biocompatibility have been successfully applied to measure intracellular pH in the pH range of 4鈥? and estimate pH fluctuations associated with different stimuli in cells. Moreover, we expect that this self-assembled approach is applicable to the construction of nanoprobes for other targets in cells just by replacing the respective indicator dyes with relevant indicators.