文摘
In this paper, the use of supercritical fluid extraction (SFE) and micellar electrokinetic capillarychromatography (MEKC) is proposed for the complete analysis of volatile nitrosamines in sausages.The extraction fluid used was COB>2 and variables such as density, temperature of thimbles, extractiontime, modifier, fluid flow, and kind of traps were investigated. Several experiments were carried outto obtain the most favorable conditions for analysis of volatile nitrosamines in sausages. The recoveriesranged from 21 to 82% for the five nitrosamines studied. The optimal condition of extraction was 0.2g of sample fortified with 10 mg/kg, using dynamic extraction during 20 min and with adsorbent Florisilin the trap. The solvent selected for the elution of the analytes was methanol.