文摘
This work presents Raman spectra obtained from thinfilms of protein materials which are commonly used asbinding media in painted works of art. Spectra wererecorded over the spectral range of 3250-250 cm-1,using an excitation wavelength of 785 nm, and severalbands have been identified in the fingerprint region thatcorrespond to the various proteins examined. Differencesin the C-H vibrations located between 3200 and 2700cm-1 can be accounted for with reference to the aminoacid composition of the protein-based binding media aswell as the presence of fatty acid esters, in the case of eggyolk. In addition, the discrimination of different proteinson the basis of variations in spectra between 3200 and2700 cm-1 can be achieved following multivariate analysisof a large data set of spectra, providing a novel andnondestructive alternative based on Raman spectroscopyto other methods commonly used for the analysis ofproteins.