Structural Mass Spectrometry: Rapid Methods for Separation and Analysis of Peptide Natural Products

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• 作者：Cody R. Goodwin ; Larissa S. Fenn ; Dagmara K. Derewacz ; Brian O. Bachmann ; John A. McLean
• 刊名：Journal of Natural Products
• 出版年：2012
• 出版时间：January 27, 2012
• 年：2012
• 卷：75
• 期：1
• 页码：48-53
• 全文大小：353K
• 年卷期：v.75,no.1(January 27, 2012)
• ISSN：1520-6025

文摘

A significant challenge in natural product discovery is the initial discrimination of discrete secondary metabolites alongside functionally similar primary metabolic cellular components within complex biological samples. A property that has yet to be fully exploited for natural product identification and characterization is the gas-phase collision cross section, or, more generally, the mobility鈥搈ass correlation. Peptide natural products possess many of the properties that distinguish natural products, as they are frequently characterized by a high degree of intramolecular bonding and possess extended and compact conformations among other structural modifications. This report describes a rapid structural mass spectrometry technique based on ion mobility鈥搈ass spectrometry for the comparison of peptide natural products to their primary metabolic congeners using mobility鈥搈ass correlation. This property is empirically determined using ion mobility鈥搈ass spectrometry, applied to the analysis of linear versus modified peptides, and used to discriminate peptide natural products in a crude microbial extract. Complementary computational approaches are utilized to understand the structural basis for the separation of primary metabolism derived linear peptides from secondary metabolite cyclic and modified cyclic species. These findings provide a platform for enhancing the identification of secondary metabolic peptides with distinct mobility鈥搈ass ratios within complex biological samples.