Inhibition of Fibrillar Assemblies of l-Phenylalanine by Crown Ethers: A Potential Approach toward Phenylketonuria

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• 作者：Debasis Banik ; Rupam Dutta ; Pavel Banerjee ; Sangita Kundu ; Nilmoni Sarkar
• 刊名：Journal of Physical Chemistry B
• 出版年：2016
• 出版时间：August 11, 2016
• 年：2016
• 卷：120
• 期：31
• 页码：7662-7670
• 全文大小：800K
• 年卷期：0
• ISSN：1520-5207

文摘

In this article, our aim is to investigate the interaction of l-phenylalanine (l-Phe) fibrils with crown ethers (CEs). For this purpose, two different CEs (15-Crown-5 (15C5) and 18-Crown-6 (18C6)) were used. Interestingly, we have observed that both CEs have the ability to arrest fibril formation. However, 18C6 was found to be a better candidate compared to 15C5. Field emission scanning electron microscopy and fluorescence lifetime imaging microscopy were used to monitor the fibril-arresting kinetics of CEs. The arresting process was further confirmed by fluorescence correlation spectroscopy and nuclear magnetic resonance studies.