Photodynamic Properties of a Bispyrrolecarboxamide-Modified Hypocrellin B: The Role of Affinity and Ascorbic Acid
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- 作者：Yanyan Liu ; Qianxiong Zhou ; Zhanghua Zeng ; Rui Qiao ; Xuesong Wang ; Baowen Zhang
- 刊名：Journal of Physical Chemistry B
- 出版年：2008
- 出版时间：August 14, 2008
- 年：2008
- 卷：112
- 期：32
- 页码：9959-9965
- 全文大小：197K
- 年卷期：v.112,no.32(August 14, 2008)
- ISSN：1520-5207
文摘
The photodynamic properties of a new hypocrellin B (HB) derivative bearing a bispyrrolecarboxamide pendant (HB-Net) were investigated in detail. EPR experiments revealed that the generation ability of reactive oxygen species (ROS) of HB-Net is inferior to that of HB. DNA melting temperature measurements and ethidium bromide (EB) displacement assay illustrated the affinity of HB-Net toward dsDNA as the result of the bispyrrolecarboxamide unit, which is structurally related to the well-known minor groove binder netropsin. The radical generation abilities of HB and HB-Net can be enhanced by ascorbic acid via the photoinduced electron transfer from ascorbic acid to the photosensitizer, however, only the DNA photodamage capability of HB-Net can be improved significantly by ascorbic acid due to the stronger affinity of HB-Net to DNA. Consequently, the combination of HB-Net and ascorbic acid may lead to efficient DNA photodamage even in anaerobic conditions.